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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning <t>microscopy</t> (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).
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Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning microscopy (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).

Journal: Materials Today Bio

Article Title: The utilization of a magneto-fluorescence sequential labeling strategy for the visualization and tracking of intestinal translocation of oral microbiota

doi: 10.1016/j.mtbio.2026.103062

Figure Lengend Snippet: Sequential labeling strategy of MSP@PAA-ADA and Cy5ADA. (A) Schematic steps of MSP@PAA-ADA and Cy5ADA sequential labeling strategy. (B) The magnified view showing the material adsorbed around the magnet. (C) Gram staining, (D) TEM images, and (E) confocal laser scanning microscopy (CLSM) images of the collected gut microbiota after magnetic separation. Scale bar is 100 μm (C, E) and 200 nm (D).

Article Snippet: Scanning Electron Microscopy images (SEM, Hitachi S-4800) were obtained using a scanning electron microscope at an accelerating voltage of 20 kV.

Techniques: Labeling, Staining, Confocal Laser Scanning Microscopy